Megabead NIST Traceable Particle Size Standards

• Single Particle Optical Sensing (SPOS) is a combination of light extinction and light scattering.

• Individual particles pass through a small optical sensing region one at a time and produce a detecting pulse.

• The magnitude of the pulse depends on the size of the particle.

• The particle size distribution plot of the sample is constructed one particle at a time by comparing the detected pulse heights with a standard calibration curve based on NIST traceable particles of known diameters.