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Polyethylenimine(PEI)

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  • Apperance

Linear Polyethylenimine

: Solid / Free Flowing Solid / Liquid

Cat# 23966

  • Packaging Size :   1 g

  • Formular :   (CH₂CH₂NH)n

  • Molecular Weight :   25,000

  • Soluble In :   hot water, cold water at low pH, methanol, ethanol

  • Insoluble In :   benzene, ethyl ether, acetone

  • Appearance :   white to yellow solid

PEI 25K is a powerful, trusted, and cost-effective transient transfection reagent. In HEK293 and CHO expression systems, PEI offers consistently high gene expression on a wide scale (96 well plates up to 100 L bioreactors). Relative to most other options, using PEI to prepare transfection reagents in-house can offer as much as a 40% reduction in total transfection costs.

Polyethylenimine, Linear, MW 2,500 (PEI 2500)

Cat# 24313

  • Packaging Size :   2 g

  • Formular :   (CH₂CH₂NH)n

  • Molecular Weight :   2,500

  • Soluble In :   hot water, cold water at low pH, methanol, ethanol

  • Insoluble In :   benzene, ethyl ether, acetone, cold water

  • Appearance :   tan to brown solid

Linear polyethylenimine (L-PEI 2500) is a high-charge cationic polymer that readily binds highly anionic substrates. Industrially, linear PEI can improve the appearance of negatively charged dyes by modulating their properties and improving their adherence to surfaces.

L-PEI 2500 contains 7-11% of the acrylated amine from isomerization-polymerization of 2-ethyl-2-oxazoline followed by acid-hydrolysis of resulting N-propionyl-PEI.

PEI MAX - Transfection Grade Linear Polyethylenimine Hydrochloride (MW 40,000)

Cat# 24765

  • Packaging Size :   1 g

  • Molecular Weight :   40,000 (~22,000 free base)

  • Soluble In :   cold and room temperature water

  • Insoluble In :   common organic solvents (ethanol, acetone, tetrahydrofuran)

  • Appearance :   white to off-white free flowing solid

PEI MAX 40K (also known as PEI 22K in free base) is a powerful, trusted, and cost-effective transient transfection reagent. In HEK293 and CHO expression systems, PEI offers consistently high gene expression on a wide scale (96 well plates up to 100 L bioreactors). Each year, more researchers and companies turn to Polysciences PEI to gain an edge in their critical work. Relative to most other options, using PEI to prepare transfection reagents in-house can offer as much as a 40% reduction in total transfection costs

PEI MAX 40K is easier to use and offers consistently higher titers than PEI 25K. PEI 25K transfection solutions typically take several hours to prepare, while PEI MAX 40K can be converted to a ready-to-use solution in under two hours. Additionally, PEI 25K contains 4-11% residual propionyl groups, which prevents the polymer backbone from strongly binding to DNA. PEI MAX 40K’s fully depropionylated structure means each batch performs consistently higher.

Cat# 26008

  • Packaging Size :   5 ml / 50 ml

  • Appearance :   liquid

Transporter 5™ is a premium ready-to-use transfection reagent prepared from our popular linear polyethylenimine MAX (PEI MAX 1 mg/mL). Transporter 5™ effectively transfects mammalian and insect cells, especially HEK-293, CHO, and Sf9.

We understand every transfection is a major investment, so we designed Transporter 5™ to be a reliable reagent in any process.

The quality of Transporter 5™ begins with our production process. Our special production method for PEI creates pure polymer with no batch-to-batch molecular weight variation. Transporter 5™ combines our consistent PEI with the highest quality USP/NF reagents in sterile, single-use equipment. While standard methods use 0.2µm sterile-filters, we use 0.1µm to completely eliminate mycoplasma risk.

Our Quality Control ensures Transporter 5™ is the most reliable reagent available. We developed our own scientifically-sound, quantitative performance assay for Transporter 5™. Most commercial performance assays are based on qualitative assays like SEAP and GFP. These assays cannot ensure the high titers most scientists require. The few reagents that are tested quantitatively typically use undisclosed HDAC inhibitors to improve results. Transporter 5™ is required to produce high titers without any supplementation. Even after initial quality control, we continuously monitor performance to ensure each batch is as good as when it was created.