the future of life science,

make the better tomorrow with CellKor

covidpage3.png

Tools for SARS-CoV-2 Research 

The coronavirus, named for its coronal form, was first identified in the 1960s and can cause a range of respiratory symptoms. Coronavirus such as Middle East respiratory syndrome coronavirus (MERS-CoV) and severe acute respiratory syndrome coronavirus (SARS-CoV) can aggravate human symptoms and cause pneumonia, severe acute respiratory syndrome and death. SARS-CoV-2 (2019-nCoV) is the newly discovered coronavirus strain.

Chinese scientists have determined that novel coronavirus belongs to 2B CoV subgroup and is different from sars-cov. Genome sequencing showed that the sequence homology of SARS-CoV-2 and SARS-CoV was 79.5%, and that of SARS-CoV-2 and bat coronavirus was 96% [1].

Coronaviruses invade host cells by attaching spike proteins to specific host cell receptors. SARS-CoV uses the angiotensin-converting enzyme II(ACE2) host cell receptor, while MERS-CoV uses CD26(DPP4) [2]. Recent results indicate that SARS-CoV-2 can enter cells using the same receptor ACE2 as SARS-CoV [1.3.4]. More studies are needed to confirm ACE2 is the only way to infect the host for SARS-CoV-2. After SARS-CoV-2 enters the cell through ACE2, Cathepsin degrades Spike protein to release nucleic acid. Some studies have shown that using Cathepsin B/L inhibitors can block the process. The study also showed that SARS-CoV-2 could also be directly hydrolyzed by the transmembrane protein TMPRSS2, enter and release nucleic acids [5]。AI was used to screen the inhibitors (Baricitinib, Sunitinib, Erlotinib, etc.) of host endocytic regulatory factor AAK1, the inhibitors can block the delivery of virus to cells and the intracellular assembly of virus particles by inhibiting AAK1[6]。

FineTest® can provide a variety of tools for coronavirus (SARS-CoV-2) research, including human anti- SARS-CoV-2 antibody (IgG, IgM), inflammatory cytokines, to help the scientific community understand the mechanisms of infection and develop effective treatments.

SARS-CoV-2 specific antibodies, proteins and ELISA Kits 

Catalog No.
Product Name
Application
EH4934
2019-Novel Coronavirus(2019-nCoV) RT-qPCR Detection Kit
RT-qPCR Detection Kit
EH4933
2019-nCoV IgM IgG Antibody Colloidal Gold Assay Kit
Colloidal Gold Assay Kit
EH4940
Human anti-SARS-CoV2 IgM ELISA Kit (quantitative)
ELISA
EH4397
Human anti-SARS-CoV2 IgG ELISA Kit (quantitative)
ELISA
EH4396
Human anti-2019 nCoV(N) IgM ELISA Kit (qualitative)
ELISA
EH4395
Human anti-2019 nCoV(N) IgG ELISA Kit (qualitative)
ELISA
FNSA-0113
poly-HRP conjugated Mouse anti-Human IgM
ELISA
FNSA-0114
poly-HRP conjugated Mouse anti-Human IgG
ELISA
FNab10306
Mouse anti-Human IgM
ELISA, IHC, WB, Colloidal Gold-Based
FNab10307
Mouse anti-Human IgG
ELISA, IHC, WB, Colloidal Gold-Based
FNab10309
anti-2019 nCOV N protein antibody (clone 3C6)
ELISA, Colloidal Gold-Based, WB
P3003
Recombinant Nucleoprotein [2019-Novel Coronavirus]
ELISA, Western Blot (WB)
FNab10308
anti-2019 nCOV N protein antibody (clone 1G5)
ELISA, Colloidal Gold-Based, WB

References 

  1. Shi, Z.L., et al. Discovery of a novel coronavirus associated with the recent pneumonia outbreak in humans and its potential bat origin. bioRxiv 2020.

  2. Xintian Xu et al. Evolution of the novel coronavirus from the ongoing Wuhan outbreak and modeling of its spike protein for risk of human transmission. SCIENCE CHINA Life Sciences.

  3. Xu, X., et al. Evolution of the novel coronavirus from the ongoing Wuhan outbreak and modeling of its spike protein for risk of human transmission. Sci. China Life Sci. 2020.

  4. Letko, M.C., Munster, V. Functional assessment of cell entry and receptor usage for lineage B β-coronaviruses, including 2019-nCoV. bioRxiv 2020.