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COVID-19 Response

Single-Use Dry Reagents for monitoring of Immune System

Although our company is not able to provide SARS-CoV-2 screening assays (e.g. molecular RT-qPCR assays or serological tests to identify IgM and IgG antibodies to the coronavirus), we are proud to partner with group of Czech medical faculty hospitals to support healthcare professionals efforts in better understanding the COVID-19 patient immune system status, which can possibly aid to the management of medical intervention.

We developed products for multicolor flow-cytometric immunophenotyping of cells of the human immune system from peripheral blood. Design of the new products reflects the recently published report on the kinetics of immune responses in relation to clinical and virological features of a COVID-19 patient that required hospitalization (1) and other studies observing immunologic features or pathological findings in severe COVID-19 patients (2, 3).

Monoclonal antibody conjugate multicolor panels are designed to enable characterization of antibody secreting cells (using DryFlowEx ASC Screening Kit) and subpopulations of activated T cells, including circulating follicular helper cells (using DryFlowEx ACT T Screening Kit) in EDTA anticoagulated peripheral blood.

In order to minimize hands-on-time and contacts with the infectious blood specimen, the reagents are provided in a ready-to-use single-test tube format. Each tube (standard 12 x 75 mm flow cytometry tube) contains a layer with the dried pre-formulated mixture of antibody conjugates for direct labeling of target cells.

Simple laboratory workflow enables operator to follow just three easy steps:

  • add whole blood specimen to the test tube, vortex and incubate;

  • lyse erythrocytes with EXCELLYSE Easy reagent and wash;

  • acquire sample and get cytometry data

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DryFlowEx ASC Screen Kit (ED7704)

  • Application : Flow Cytometry (QC tested)

  • Description :

DryFlowEx ASC Screen Kit is designed for flow cytometry screening of antibody secreting B cells (ASC) during virus infection.

Multicolor panel of antibody conjugates CD45/ IgD/ CD27/ CD24/ CD19/ CD21/ CD38 dried in a single flow cytometry tube enables immunophenotyping of differentiated B lymphocyte subsets in EDTA-anticoagulated human blood.

  • Contents :

50 single-test tubes (ED7704-1T ASC Screen Tube); each 5 pcs of single-test tubes are packed with dessicant in moisture-protective aluminum pouches (ED7704-5T ASC Screen 5T Pack)

1 set of compensation tubes (ED7704-CT ASC Screen Compensation Tubes Pack)
ED7704-CT1 CD45 Pacific Blue™
ED7704-CT2 IgD FITC
ED7705-CT3 CD27 PE
ED7704-CT4 CD24 PerCP-Cy5.5
ED7704-CT5 CD19 PE-Cy7
ED7704-CT6 CD21 APC
ED7704-CT7 CD38 APC-Cy7
Identical fluorochrome-labeled antibodies allow easy and accurate compensation procedure.

act.png

DryFlowEx ACT T Screen Kit (ED7705)

  • Application : Flow Cytometry (QC tested)

  • Description :

DryFlowEx ACT T Screen Kit is designed for flow cytometry screening of activated T cells (ACT T) during virus infection.

Multicolor panel of antibody conjugates CD45/ CD8/ CD4/ PD-1/ HLA-DR/ CD3/ CXCR5/ CD38 dried in a single flow cytometry tube enables immunophenotyping of activated T lymphocyte subsets including a specific population of circulating follicular T helper cells in EDTA-anticoagulated human blood.

  • Contents :

50 single-test tubes (ED7705-1T ACT T Screen Tube); each 5 pcs of single-test tubes are packed with dessicant in moisture-protective aluminum pouches (ED7705-5T ACT T Screen 5T Pack)

1 set of compensation tubes (ED7705-CT ACT T Screen Compensation Tubes Pack)
ED7705-CT1 CD4 FITC
ED7705-CT2 PD-1 (CD279) PE
ED7705-CT3 HLA-DR PerCP-Cy™5.5
ED7705-CT4 CD3 PE-Cy™7
ED7705-CT5 CXCR5 (CD185) APC
ED7705-CT6 CD38 APC-Cy™7
ED7705-CT7 CD45 Pacific Blue™
ED7705-CT8 CD8 Pacific Orange™
Identical fluorochrome-labeled antibodies allow easy and accurate compensation procedure.

EXCELLYSE Easy (ED7066)

  • Application : Flow Cytometry (QC tested)

  • Description :

The EXCELLYSE Easy 100 ml lysing solution is intended for red blood cell lysis following antibody staining of human peripheral blood leukocytes. The lysing solution is appropriate for use with EXBIO single colour monoclonal antibodies and KOMBITEST reagents and can be used in both lyse/wash and lyse/no wash protocol.

  • Contents :

EXCELLYSE Easy lysing solution is a 10× concentrated reagent provided as 100 ml package. This quantity is sufficient for 1000-2000 tests. The reagent must be diluted 1:10 with deionized water prior to use.

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Tel. 02.6951.5302  I  Fax. 02.6951.5307  I  info@cellkor.com

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